Invitrogen™ Human CDK9/Cyclin T1, His Tag Recombinant Protein

Product Code. 11513627

Brand: Invitrogen™ PV4131

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Recombinant, Full Length.

Cyclin-dependent kinase 9 (CDK9) and Cyclin T1 form the positive transcription elongation factor b (p-TEFb) complex, which is crucial for transcriptional regulation and mRNA maturation. CDK9 is a transcriptional kinase expressed widely across human tissues, with notably higher levels in terminally differentiated cells, influencing transcriptional elongation by phosphorylating the RNA polymerase II C-terminal domain. This kinase's gene is mapped to chromosome 9q34.1 and produces two isoforms through two distinct promoters. CDK9, when coupled with Cyclin T1, modulates oncogene expression and activity, representing essential roles in cell survival and proliferation. The dysregulation of CDK9/Cyclin T1's activity has connections to tumorigenesis, highlighting their importance in cancer biology as potential therapeutic targets. Their complex operation also involves acetylation changes crucial for functional modulation. The interaction of CDK9 with other regulatory proteins emphasizes its versatile roles in cellular physiology beyond what is seen in classical cell cycle control.

Specifications

• Accession Number: O60563, P50750
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 0.02% Triton X-100, 150mM NaCl, 0.5mM EDTA, 2mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 1025, 904
• Molecular Weight (g/mol): 46.9-84.8 kDa
• Name: Human CDK9/Cyclin T1, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: C-2 k; CDC2L4; CDC2-related kinase; Cdk9; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; CTK1; cyclin dependent kinase 9; cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); PITALRE; RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK; Tat-associated kinase complex catalytic subunit
• Common Name: CDK9/Cyclin T1
• Gene Symbol: CCNT1, CDK9
• KinaseFamily: CDK and CHK Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic, Biochemical ADP accumulation
• Kinase Group: Ser⁄Thr Kinases: CMCG Group
• Shipping Condition: Dry Ice
• System Compatibility: Adapta™
• Target Entry: CDK9/Cyclin T1
• Target Gene: cyclin-dependent kinase 9
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Cell Cycle & Proliferation, Signal Transduction