Invitrogen™ Human CDK6/Cyclin D1, GST Tag Recombinant Protein

Product Code. 10503143

Brand: Invitrogen™ PV4437

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length.

Cyclin-dependent kinase 6 (CDK6) and Cyclin D1 are critical components of the cell cycle regulation, particularly in the transition from the G1 to the S phase. CDK6 is a protein kinase that requires association with cyclins, such as Cyclin D1, to become enzymatically active. This partnership is essential for phosphorylating target substrates that drive cell cycle progression. CDK6 and Cyclin D1 together form complexes that regulate the retinoblastoma protein (Rb) pathway, which is a key mechanism controlling cell proliferation. The expression and activation of CDK6/Cyclin D1 complexes are tightly regulated, and imbalances can contribute to oncogenesis. Structural studies have shown that CDK6 has a unique C-terminal region crucial for its nuclear transport and localization, which is essential for its function. Cyclin D1's dynamics-its expression, accumulation, and degradation-are pivotal in its regulatory roles, especially its interactions with CDK4 and CDK6. In cancer, genetic alterations affecting CDK6 and Cyclin D1 can lead to uncontrolled cell division, emphasizing their significance in cancer progression and as potential therapeutic targets.

Specifications

• Accession Number: P24385, Q00534
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 150mM NaCl, 0.5mM EDTA, 2mM DTT, 0.02% Triton X-100, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 1021, 595
• Molecular Weight (g/mol): 66.4-61.2 kDa
• Name: Human CDK6/Cyclin D1, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 5830411I20; AI504062; CDK6; CDKN6; Cell division protein kinase 6; CR2 protein kinase; Crk2; cyclin dependent kinase 6; cyclin-dependent kinase 6; MCPH12; MGC59692; PLSTIRE; Serine/threonine-protein kinase PLSTIRE
• Common Name: CDK6/Cyclin D1
• Gene Symbol: CCND1, CDK6
• KinaseFamily: CDK and CHK Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Kinase Group: Ser/Thr Kinases: CMCG Group
• Shipping Condition: Dry Ice
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length
• Form: Liquid
• Research Category: Drug Development