Invitrogen™ CDK4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA527827

Product Code. 13481967

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, PC-12. Predicted reactivity: Mouse (95%), Rat (94%), Pig (98%), Sheep (96%), Rhesus Monkey (100%), Bovine (96%).

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

Specifications

• Antigen: CDK4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cdk4
• Gene Alias: Cdk4; Cell division protein kinase 4; CMM3; Crk3; cyclin dependent kinase 4; cyclin-dependent kinase 4; MGC14458; p34>PSK-J3>/cdk4; PSK-J3; serine/threonine kinase; Unknown (protein for MGC:133903)
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1019, 12567, 510618, 94201
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.82 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P11802, P30285, P35426, Q32KY4
• Gene Symbols: Cdk4
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG