Invitrogen™ Human CDK13/cyclin K, GST Tag, His Tag Recombinant Protein

Product Code. 17108825

Brand: Invitrogen™ A33569

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This CDK13/cyclin K recombinant human protein (CDK13: 656-1050, cyclin K 1-300) was expressed in insect cells. CDK13/cyclin K recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. Validated for use with the LanthaScreen™ Eu Kinase Binding assay Available in multiple pack sizes for convenient use Also available in our SelectScreen Kinase Profiling Service Protein Form: Recombinant, Active, Catalytic The gene accession number for CDK13 and cyclin K are NP_003709.3 and NP_001092872.1.

Cyclin-dependent kinase 13 (CDK13) and Cyclin K form a complex that is integral to RNA processing and transcription regulation. CDK13, a member of the CDK family, is involved in the modulation of gene expression through phosphorylation of the C-terminal domain of RNA Polymerase II, affecting the transcription elongation phase. Cyclin K acts as a regulatory partner to CDK13, stabilizing its active conformation to efficiently catalyze its kinase functions. CDK13, along with Cyclin K, plays a pivotal role in RNA metabolism, influencing RNA splicing and 3' end processing, which are crucial for the synthesis of mature mRNA. Structural studies have characterized CDK13 as having unique features differentiating it from other CDKs, including distinctive domain arrangements that enable interaction with Cyclin K. The dysregulation of CDK13/Cyclin K activity has been observed in various diseases, including cancer, pointing to their importance as therapeutic targets. Research also highlights CDK13's involvement in maintaining cellular homeostasis, underscoring the complex network of interactions this kinase engages in beyond transcriptional control.

Specifications

• Accession Number: O75909, Q14004
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM HEPES with 15mM glutathione, 5mM DTT, 100mM NaCl, 20% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 8621, 8812
• Molecular Weight (g/mol): 73.8-63.0 kDa
• Name: Human CDK13/cyclin K, GST Tag, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 2310015O17Rik; CDC2L; Cdc2l5; CDC2-related protein kinase 5; CDK13; cell division cycle 2-like 5 (cholinesterase-related cell division controller); cell division cycle 2-like protein kinase 5; Cell division protein kinase 13; CHED; Cholinesterase-related cell division controller; cyclin dependent kinase 13; cyclin-dependent kinase 13; hCDK13; Kiaa1791
• Common Name: CDK13/cyclin K
• Gene Symbol: CCNK, CDK13
• KinaseFamily: CDKs and CHKs
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: CDK13: 656 -1050, Cyclin K: 1-300
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Active, Catalytic
• Form: Liquid