Invitrogen™ Human CDK1/Cyclin B, His Tag Recombinant Protein

Product Code. 15448423

Brand: Invitrogen™ PR4768C

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Recombinant, Full Length.

Cyclin-dependent kinase 1 (CDK1) and its regulatory partner Cyclin B are fundamental to the control of cell cycle transitions, specifically the G2/M phase transition, which ultimately leads to cell division. CDK1, a serine/threonine kinase, forms a complex with Cyclin B necessary for the initiation of mitosis. This complex phosphorylates various substrates to facilitate structural changes required for mitosis. The CDK1/Cyclin B complex is tightly regulated through a series of phosphorylation and dephosphorylation events that control its activation state. The nuclear-cytoplasmic shuttling of Cyclin B1, coupled with CDK1 activity, is crucial for proper mitotic entry and progression. Interestingly, CDK1/Cyclin B1 also plays a role in the coordination of mitochondrial bioenergetics, indicating a broader role in cell metabolism and division. Allosteric modifications, such as acetylation of specific lysine residues, notably lysine-33, also influence CDK1/Cyclin B interaction and function, emphasizing the intricate regulatory mechanisms governing their activity.

Specifications

• Accession Number: P06493, P14635
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 20mM tris with 0.5mM EDTA, 2mM DTT, 150mM NaCl, 0.01% Triton X-100, 20% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 891, 983
• Molecular Weight (g/mol): 91 kDa
• Name: Human CDK1/Cyclin B, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 5363; ABL1; cdc; CDC2; cdc-2; cdc2 kinase; CDC28A; Cdc2a; cdc2-a; cdc2Dm; cdc2 x 1.1; CDCDm; CDK1; Cdk-1; cdk1.S; CDK1/CDC2; CDK1-A; cdk1-b; Cdk1-PA; CDKN1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; cell division control; cell division control protein 2 homolog; Cell division control protein 2 homolog 1; cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2 A; cell division protein kinase 1; Cell division protein kinase 1-A; CG5363; CG5363-PA; cyclin dependent kinase; cyclin dependent kinase 1; cyclin-dependent kinase; Cyclin-dependent kinase 1; cyclin-dependent kinase 1 S homeolog; cyclin-dependent kinase 1-A; D2366; Dcdc2; dCdk1; DKFZp686L20222; Dm cdc2; Dmcdc2; DmCdk1; Dmel\CG5363; Dmel_CG5363; DNA polymerase delta catalytic subunit; DNA polymerase III; DNA-directed DNA polymerase delta POL3; group 4; HPR6; KCIP-1; l(2)31 Eh; LD38718p; maturation-promoting factor; MGC111195; MGC53829; MPF; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; p34cdc2; p34-cdc2 homologue; POL3; Protein 1054; protein 2; PSTAIR; TE x 1; unnamed protein product; xcdc2; XELAEV_18036802mg; YDL102W
• Common Name: CDK1/Cyclin B
• Gene Symbol: CCNB1, CDK1
• KinaseFamily: CDK and CHK Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Kinase Group: Ser⁄Thr Kinases: CMCG Group
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Research Category: Cell Cycle & Proliferation, Signal Transduction
• Purity or Quality Grade: 80% by SDS-PAGE