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Eurofins DiscoverX Cdk1/cyclin B Protein, Active

Product Code. 17380184
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250 μg
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17380184 10 μg 10µg
17390184 250 μg 250µg
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Product Code. 17380184 Supplier Eurofins DiscoverX Supplier No. 14450

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Description

Active, C-terminal His6-tagged human full length Cdk1, and N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

The cyclin-dependent kinases (CDKs), a subfamily of protein kinases, promote cell cycle progression. In mammals, nine different CDKs (cdk 1 to cdk 9; cdk 1 is better known as cdc 2) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk1 induces entry into M-phase of the cell cycle in eukaryotes.

GenBank NM_001786 / EMBL M25753

Specifications

Formulation mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Molecular Weight (g/mol) 35 kDa (CDK1) and 75 kDa (Cyclin B)
Quantity 10 μg
Species Human
Recombinant Recombinant
Protein Tag C-terminal 6His / N-terminal GST
Expression System Recombinant enzyme expressed in Sf21 insect cells
Protein Form Full-length
Purity or Quality Grade Combined ≥63%
Protein CDK1/Cyclin B, active
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