Invitrogen™ Human CDK1/cyclin A2, GST Tag Recombinant Protein

Product Code. 15448933

Brand: Invitrogen™ PV6280

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant The gene accession number for the subunits (CDK1/Cyclin A2) are NP_001777.1 and NP_001228.1.

CDK1, or Cyclin-Dependent Kinase 1, is a critical member of the cyclin-dependent kinase family that regulates cell cycle progression. CDK1 is essential for the transition from the G2 phase to the M phase and for the progression through mitosis. Cyclin A2 is a regulatory subunit that binds to CDK1 and activates its kinase activity. The binding of Cyclin A2 to CDK1 is necessary for the proper functioning of CDK1, as cyclins regulate the activation and specificity of CDKs. Together, the CDK1/Cyclin A2 complex plays a vital role in the regulation of the cell cycle, particularly during the S phase and the G2/M transition. This complex is involved in the phosphorylation of key substrates required for DNA replication, the initiation of mitosis, and the proper alignment and separation of chromosomes, ensuring accurate and orderly cell division.

Specifications

• Accession Number: P06493, P20248
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.1mM PMSF, 0.1mM EDTA, 150mM NaCl, 25% glycerol, 0.25mM DTT, 0.1mM EGTA and no preservative; pH 7.5
• Gene ID (Entrez): 890, 983
• Molecular Weight (g/mol): 60.4-74.8 kDa
• Name: Human CDK1/cyclin A2, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 5363; ABL1; cdc; CDC2; cdc-2; cdc2 kinase; CDC28A; Cdc2a; cdc2-a; cdc2Dm; cdc2 x 1.1; CDCDm; CDK1; Cdk-1; cdk1.S; CDK1/CDC2; CDK1-A; cdk1-b; Cdk1-PA; CDKN1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; cell division control; cell division control protein 2 homolog; Cell division control protein 2 homolog 1; cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2 A; cell division protein kinase 1; Cell division protein kinase 1-A; CG5363; CG5363-PA; cyclin dependent kinase; cyclin dependent kinase 1; cyclin-dependent kinase; Cyclin-dependent kinase 1; cyclin-dependent kinase 1 S homeolog; cyclin-dependent kinase 1-A; D2366; Dcdc2; dCdk1; DKFZp686L20222; Dm cdc2; Dmcdc2; DmCdk1; Dmel\CG5363; Dmel_CG5363; DNA polymerase delta catalytic subunit; DNA polymerase III; DNA-directed DNA polymerase delta POL3; group 4; HPR6; KCIP-1; l(2)31 Eh; LD38718p; maturation-promoting factor; MGC111195; MGC53829; MPF; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; p34cdc2; p34-cdc2 homologue; POL3; Protein 1054; protein 2; PSTAIR; TE x 1; unnamed protein product; xcdc2; XELAEV_18036802mg; YDL102W
• Common Name: CDK1/Cyclin A2
• Gene Symbol: CCNA2, CDK1
• Protein Length: 1-297
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-297
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Serine/Threonine Kinase
• Target Entry: CDK1/cyclin A2
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid