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Eurofins DiscoverX CDC7/Cyclin B1 Protein, Active
Description
CDC7 is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity. Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Additional transcript sizes have been detected, suggesting the presence of alternative splicing, Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition.Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3.
Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1.
GenBank NM_003503.2 / GenBank NM_031966.3
Specifications
Specifications
| Formulation | mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Molecular Weight (g/mol) | 92 kDa (CDC7) and 75 kDa (Cyclin B1) |
| Quantity | 10 μg |
| Species | Human |
| Recombinant | Recombinant |
| Protein Tag | N-terminal GST / N-terminal GST |
| Expression System | Recombinant enzyme expressed in Sf21 insect cells |
| Protein Form | Full-length |
| Purity or Quality Grade | ≥61% |
| Protein | CDC7/Cyclin B1, active |
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