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Invitrogen™ Cdc27 Monoclonal Antibody (AF3.1)

Product Code. 11554782
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. Quantity unitSize
11554782 100 μL 100µL
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Product Code. 11554782 Supplier Invitrogen™ Supplier No. MA124749

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Description

Mouse Monoclonal Antibody

Recommended positive controls: HeLa nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Cdc27
Applications ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone AF3.1
Concentration 2.3 mg/mL
Conjugate Unconjugated
Formulation PBS with 15mM sodium azide; pH 7.4
Gene CDC27
Gene Accession No. A2A6Q5, A7Z061, P30260, Q4V8A2
Gene Alias AI452358; AN CDC27Hs; ANAPC3; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; BC023187; cdc27; CDC27 homolog; cdc27.S; CDC27Hs; cell division cycle 27; cell division cycle 27 homolog; cell division cycle 27 homolog (S. cerevisiae); cell division cycle 27 S homeolog; cell division cycle protein 27 homolog; D0S1430E; D17S978E; HNUC; H-NUC; hypothetical protein; NUC2; nuc2 homolog; XELAEV_18046079mg
Gene Symbols CDC27, cdc27.S
Host Species Mouse
Immunogen Synthetic peptide, conjugated to KLH, corresponding to residues 814-823 of human Cdc27.
Purification Method Purified
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 217232, 360643, 443994, 490924, 540660, 996
Target Species Bovine, Canine, Human, Mouse, Rat, Frog
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG2b
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