Invitrogen™ V5 Tag Recombinant Mouse Monoclonal Antibody (TCM5), Alexa Fluor™ Plus 555

Mouse Recombinant Monoclonal Antibody

Brand:  Invitrogen™ 740058MP555

Product Code. 30275360

Description

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This TCM5 antibody has been tested by western blot, immunocytochemistry and flow cytometric analysis of HEK-293E cells transiently transfected with V5 constructs. This may be used for immunocytochemistry at 5 μg/mL and for flow cytometry at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully...

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Specifications

• Antigen: V5 Tag
• Classification: Recombinant Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Host Species: Mouse
• Quantity: 50 μL
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: TCM5
• Conjugate: Alexa Fluor Plus 555
• Gene Alias: simian virus 5 antibody; SPV5gp2; sv5; V5; v-5; V5 Epitope Tag
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG2b κ