CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), FITC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 11-0083-81

Product Code. 15208019

Description

Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells. Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.

Specifications

• Antigen: CD8b
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P10300
• Gene Symbols: Cd8b1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12526
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioH35-17.2 (H35-17.2)
• Conjugate: FITC
• Gene: Cd8b1
• Gene Alias: Cd8b1
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.