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CD8a, eVolve 655, clone: 53-6.7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 86-0081-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15540827

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-6.7 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

CD8a
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P01731
CD8, Leu-2, MAL, p32, CD8a
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
53-6.7
eVolve 655
Cd8a
Conjugated
Cd8a
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Primary
12525
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