CD8a Monoclonal Antibody (YCATE55.9), Super Bright™ 600, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15963544	Affymetrix eBioscience 63-5080-41	25 Tests
15973544	Affymetrix eBioscience 63-5080-42	100 Tests

Description

Description: This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha. This cell surface receptor is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). In dogs, CD8 alpha is expressed on thymocytes, a subset of mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs). CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation. This monoclonal antibody has been reported to inhibit cytotoxic T cell generation and function in vitro. In addition, this antibody has been reported to be immunosuppressive in vivo. Applications Reported: This YCATE55.9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YCATE55.9 antibody has been pre-diluted and tested by flow cytometric analysis of normal canine peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

CD8a Monoclonal antibody specifically detects CD8a in Canine samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD8a
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at
• Gene Symbols: Cd8a, CD8B
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: YCATE55.9
• Applications: Flow Cytometry
• Conjugate: Super Bright 600
• Host Species: Rat
• Target Species: Canine
• Gene Accession No.: P33706
• Gene ID (Entrez): 403157, 483076
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody