Invitrogen™ CD88 (C5aR1) Monoclonal Antibody (S5/1), Functional Grade, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 16-0888-85

Product Code. 17141092

Description

Description: This S5/1 monoclonal antibody recognizes human CD88 also known as C5aR1. C5aR1 is a complement receptor widely expressed on cells of the myeloid lineage. Clone S5/1 has been reported to block C5a binding. Applications Reported: This S5/1 antibody has been reported for use in flow cytometric analysis. S5/1 has also been reported for blocking activity. Applications Tested: This S5/1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.

Specifications

• Antigen: CD88 (C5aR1)
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P21730
• Gene Symbols: C5AR1
• Immunogen: BSA-Conjugated Peptide aa. 1-31.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Functional Assay
• Clone: S5/1
• Conjugate: Functional Grade
• Gene: C5AR1
• Gene Alias: anaphylatoxin C5a receptor; C5A; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a Receptor; C5AR; C5a-R; C5AR1; C5r1; Cd88; CD88 antigen; complement C5a receptor; complement C5a receptor 1; complement component 5 receptor 1; complement component 5, receptor 1; complement component 5a receptor 1; D7Msu1
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 728
• Content And Storage: 4°C, do not freeze
• Form: Liquid