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CD85d (ILT4), PE, clone: 42D1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5149-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15586996

  • £180.65 / 100 tests


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family. CD85d is a 110kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection.Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC.Applications Reported: This 42D1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 42D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD85d
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q8N423
CD85d, ILT4, LIR2, MIR10, CD85 antigen-like family member D, CD85d antigen, Ig-like transcript 4, ILT-4, ILT4CD85d, Immunoglobulin-like transcript 4, Leukocyte immunoglobulin-like receptor 2, leukocyte immunoglobulin-like receptor subfamily B member 2, leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), LIR2CD85D, LIR-2subfamily A (with TM domain), member 6, member 2, MIR10LILRA6, Monocyte/macrophage immunoglobulin-like receptor 10
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
42D1
PE
LILRB2
Conjugated
LILRB2
IgG2a
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
10288
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