CD85d (ILT4) Rat anti-Human, PE, Clone: 42D1, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-5149-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15586996

Quantity Price
1 £ 176.7 / 100 tests
Estimated Shipment date
from Supplier 08-05-2017
Add to basket


Description and Specification

Specification

Quantity 100 Tests
Isotype IgG2a
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Format Conjugated
Clone 42D1
Monoclonal or Polyclonal Monoclonal
Host Species Rat
Applications Flow Cytometry
Gene Alias LIR2, LILRB2, MIR10
Species Reactivity Human
Primary or Secondary Primary
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Conjugate PE
Regulatory Status RUO
Antigen CD85d (ILT4)
Concentration 5μL (1μg)/test

The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family. CD85d is a 110kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection.

Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC.