CD81 Monoclonal Antibody (Eat-2 (EAT2)), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0811-025

Product Code. 17171082

Description

The Eat-2 (EAT2) monoclonal antibody reacts with mouse CD81, a molecule also known as TAPA-1. CD81 is expressed on CD4^+CD8^+ thymocytes and broadly in the periphery with high level expressed by B cells, NK cells, macrophages and dendritic cells and lower level on resting T cells. Activation of T cells induces upregulation of CD81. CD81 is involved in homotypic adhesion of B and T cells as well as costimulation of T cells. The Eat-2 antibody is reported to cross-react with rat CD81. Applications Reported: The Eat-2 (EAT2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). Applications Tested: The Eat-2 (EAT2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD81 Monoclonal antibody specifically detects CD81 in Mouse samples. It is validated for Control, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot

Specifications

• Antigen: CD81
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P35762
• Host Species: Armenian Hamster
• Quantity: 25 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Clone: Eat-2 (EAT2)
• Conjugate: Functional Grade
• Gene: CD81
• Gene Alias: 26 kDa cell surface protein TAPA-1; CD 81 antigen; Cd81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); CD81 molecule; CVID6; S5.7; Tapa1; Tapa-1; Target of the antiproliferative antibody 1; Tetraspanin28; tetraspanin-28; TSPAN28; Tspan-28
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12520
• Content And Storage: 4° C
• Form: Liquid