Invitrogen™ CD81 Monoclonal Antibody (Eat-2 (EAT2)), Functional Grade, eBioscience™

Product Code.	Quantity	unitSize
15238517	100 μg	100µg

Product Code. 15238517 Supplier Invitrogen™ Supplier No. 16081182

Description

Armenian Hamster Monoclonal Antibody

Description: The Eat-2 (EAT2) monoclonal antibody reacts with mouse CD81, a molecule also known as TAPA-1. CD81 is expressed on CD4^+CD8^+ thymocytes and broadly in the periphery with high level expressed by B cells, NK cells, macrophages and dendritic cells and lower level on resting T cells. Activation of T cells induces upregulation of CD81. CD81 is involved in homotypic adhesion of B and T cells as well as costimulation of T cells. The Eat-2 antibody is reported to cross-react with rat CD81. Applications Reported: The Eat-2 (EAT2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). Applications Tested: The Eat-2 (EAT2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

Specifications

• Antigen: CD81
• Applications: Control, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: Eat-2 (EAT2)
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene: CD81
• Gene Accession No.: P35762
• Gene Alias: 26 kDa cell surface protein TAPA-1; CD 81 antigen; Cd81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); CD81 molecule; CVID6; S5.7; Tapa1; Tapa-1; Target of the antiproliferative antibody 1; Tetraspanin28; tetraspanin-28; Tspan28; Tspan-28
• Gene Symbols: CD81
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12520
• Target Species: Mouse
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG