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CD80 (B7-1) Mouse anti-Human, PE-Cyanine5, Clone: 2D10.4, eBioscience™

Mouse Monoclonal Antibody

£89.50 - £193.00

Specifications

Antigen CD80 (B7-1)
Clone 2D10.4
Target Species Human
Conjugate PE-Cyanine5
Isotype IgG1, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15587736
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Affymetrix eBioscience
15-0809-42
100 Tests
£193.00
100 tests
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Estimated Shipment
03-03-2021
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15577736
View Documents
Affymetrix eBioscience
15-0809-41
25 Tests
£89.50
25 tests
View alternative products
Save Up to 
»
Estimated Shipment
03-03-2021
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Specifications

Specifications

CD80 (B7-1)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
2D10.4
PE-Cyanine5
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.