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CD80 (B7-1), Functional Grade, clone: 2D10.4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0809-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208517

  • £68.39 / 50Āµg


Estimated Shipment
06-06-2018
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Description

Description

Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 2D10.4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD80 (B7-1)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P33681
B7-1, B7.1, Cd28l, Ly-53, Ly53, MIC17, TS/A-1, LAB7, B7.1, CD28LG, CD28LG1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryĆ¾Functional Assay
2D10.4
Functional Grade
CD80
Purified
CD80
IgG1, kappa
50μg
Store at 2-8°C.
Primary
941
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