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CD8a, Alexa Fluor 700, clone: OKT8 (OKT-8), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0086-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15580327

  • £68.27 / 25 tests


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Disclaimers:

For Research Use Only.


Description

Description

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT8 (OKT-8) antibody is offered in 2 formats: -μg size: has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.125μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5μL (0.06μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD8
OKT8 (OKT-8)
Alexa Fluor 700
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL; 5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD8 alpha, leu-2a
IgG2a
RUO
Primary
Human
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