CD75 Monoclonal Antibody (LN-1), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 50-0759-41

Product Code. 15519896

Description

Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22. Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain. Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22.Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain.Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD75
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15907
• Gene Symbols: ST6GAL1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6480
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Form: Liquid

• Applications: Flow Cytometry
• Clone: LN-1
• Conjugate: eFluor 660
• Gene: ST6GAL1
• Gene Alias: Sialyltransferase 1, SIAT1, ST6GalI, ST6N, B-cell antigen CD75, CMP-N-acetylneuraminate beta-galactosamide alpha-2, 6-sialyltransferase, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 6-sialyltransferase 1, ST6Gal I, alpha 2, 6-ST 1, beta-galactoside alpha-2, 6-sialyltransferase 1, sialyltransferase 1
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM

For Research Use Only.