CD74 Antibody (LN-2), DyLight 488, Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NBP2-33088G

Product Code. 18163483

Description

CD74 Monoclonal specifically detects CD74 in Human, Mouse, Baboon, Rat (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD74
• Classification: Monoclonal
• Conjugate: DyLight 488
• Gene Alias: CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated), CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAGgamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLADG, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia antigen-associated invariant chain, Ia-associated invariant chain, Ia-GAMMA, Ii, MHC HLA-DR gamma chain, p33
• Host Species: Mouse
• Molecular Weight of Antigen: 37 kDa
• Quantity: 0.1 mL
• Research Discipline: Immunology
• Gene ID (Entrez): 972
• Target Species: Human, Mouse, Baboon, Rat (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: LN-2
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Gene Symbols: CD74
• Immunogen: SU-DHL-4 lymphoma cells were used as the immunogen for the CD74 antibody
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: It recognizes a protein of ∼35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

For Research Use Only