CD71 (Transferrin Receptor), PerCP-eFluor™ 710, clone: R17217 (RI7 217.1.4), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-0711-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains.Applications Reported: This R17217 antibody has been reported for use in flow cytometric analysis.Applications Tested: This R17217 (RI7 217.1.4) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD71 (Transferrin Receptor)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|CD71, T9, TFR, TFR1, TR, TRFR, p90, transferrin receptor protein 1|
|R17217 (RI7 217.1.4)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.