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CD7, PerCP-eFluor™ 710, clone: 4H9, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0078-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15569066

  • £228.49 / 100 tests


Estimated Shipment date
from Supplier 28-06-2018
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Description

Description

Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation.CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma.Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD7
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P09564
GP40, LEU-9, TP41, Tp40
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
4H9
PerCP-eFluor 710
CD7
Conjugated
CD7
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
924
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