CD64 Monoclonal Antibody (10.1), APC-eFluor™ 780, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-0649-42

Product Code. 15589556

Description

Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative. Applications Reported: This 10.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

Specifications

• Antigen: CD64
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P12314
• Gene Symbols: FCGR1A
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2209
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 10.1
• Conjugate: APC-eFluor 780
• Gene: FCGR1A
• Gene Alias: AI323638; AV092959; CD64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ib receptor; Fc fragment of IgG receptor Ia; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc fragment of IgG, high affinity Ib, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc receptor, IgG, high affinity I; Fcg1; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRI; FcgammaRIa; Fcgr1; FCGR1A; Fcgr1b; FcgRI; FCRI; FLJ18345; high affinity IgG Fc receptor, subunit beta; high affinity immunoglobulin gamma Fc receptor I; high-affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I; IgG high affinity Fc receptor; IGGHAFC; RP11-196G18.2
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.