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CD64 (Fc gamma Receptor 1), APC-eFluor 780, clone: 10.1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-0649-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15589556

  • £189.69 / 100 tests


EU Stock 2
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Description

Description

Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.Applications Reported: This 10.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD64 (Fc gamma Receptor 1)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q92637, P12314
CD64, CD64A, FCRI, FLJ18345, IGFR1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
10.1
APC-eFluor 780
FCGR1B
Conjugated
FCGR1A, FCGR1B
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
2209, 2210
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