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CD63, PE-Cyanine7, clone: H5C6, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0639-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15508426

  • £77.18 / 25 tests


Estimated Shipment date
from Supplier 01-06-2018
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Description

Description

Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes. This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells. Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response.Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD63
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P08962
LAMP-3, ME491, MLA1, OMA81H, TSPAN30
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Flow Cytometry
H5C6
PE-Cyanine7
CD63
Conjugated
CD63
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
967
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