Invitrogen™ CD63 Monoclonal Antibody (446703)

Rat Monoclonal Antibody

Brand:  Invitrogen™ MA524169

Product Code. 15523862

Description

Reconstitute at 0.5 mg/mL in sterile PBS.

CD63, also known as LAMP-3, is a glycoprotein belonging to the tetraspanin family, primarily located in late endosomes, lysosomes, and secretory vesicles across various cell types. It is expressed intracellularly in resting platelets and basophils, but upon activation, CD63 translocates to the plasma membrane, making it a widely used marker for basophil activation. Unlike basophils, mast cells can expose CD63 without activation. CD63 is expressed on activated platelets, monocytes, macrophages, and granulocytes, as well as endothelial cells, fibroblasts, and smooth muscle cells. It interacts with integrins (such as VLA-3 and VLA-6) and TIMP-1, playing roles in mediating allergic responses, phagocytosis, and cell migration. Additionally, CD63 is involved in the regulation of H/K-ATPase trafficking and ROMK1 channels, and serves as a T-cell costimulation molecule. CD63 expression can be used to predict prognosis in early-stage carcinomas and is identical to the melanoma-associated antigen ME491 and the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome, highlighting its significance in both immune responses and disease processes.

Specifications

• Antigen: CD63
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P41731
• Gene Symbols: CD63
• Immunogen: HEK293 human embryonic kidney cell line transfected with mouse CD63 Met1-Met238.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen)
• Clone: 446703
• Conjugate: Unconjugated
• Gene: CD63
• Gene Alias: C75951; CD 63; Cd63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Cd63 molecule; CD63 protein; granulophysin; LAMP-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; mast cell antigen AD1; ME491; melanoma 1 antigen; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; TSPAN30; tspan-30
• Host Species: Rat
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 12512
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized