CD62L (L-Selectin) Monoclonal Antibody (OX85), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0623-80

Product Code. 15529886

Description

Description: The OX85 monoclonal antibody reacts with rat CD62L, which is also known as L-Selectin. CD62L is a 76-kDa member of the selectin family that is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. This cell surface receptor binds glycosylated, fucosylated, sulfated, and sialylated glycoproteins including CD34, GlyCAM-1, and MadCAM-1. These interactions mediate lymphocyte rolling on activated endothelium at sites of inflammation and homing to high endothelial venules of peripheral lymphoid tissues. Applications Reported: This OX85 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OX85 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD62L (L-Selectin) Monoclonal antibody specifically detects CD62L (L-Selectin) in Rat samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD62L (L-Selectin)
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P30836
• Gene Symbols: Sell
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29259
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: OX85
• Conjugate: eFluor 660
• Gene: Sell
• Gene Alias: A.11; AI528707; CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LECAM1; LECAM-1; LEU8; Leu-8; Leukocyte adhesion molecule 1; leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; L-selectin; Ly22; Ly-22; LYAM1; Lyam-1; Ly-m22; Lymph node homing receptor; lymphocyte adhesion molecule 1; lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; pln homing receptor; PLNHR; SELE; selectin E (endothelial adhesion molecule 1); selectin L; selectin L (lymphocyte adhesion molecule 1); selectin, lymphocyte; Sell; TQ1
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.