Invitrogen™ CD6 Monoclonal Antibody (IM348), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen™ 14-0061-37

Product Code. 17140412

Description

The MAb IM348 recognizes mouse CD6, a transmembrane glycoprotein of the scavenger receptor family. The extracellular domain contains three scavenger receptor cysteine rich (SRCR) domains, a transmembrane region and a cytoplasmic domain of variable length depending on the isoform. CD6 is expressed on most T lymphocytes. Expression between human and mouse is slightly different. In mice B cells in the spleen do not have CD6, while humans express it on a subset of B lymphocytes. The CD6 ligand is CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). Through interactions with CD166 or homotypic CD6-CD6, a role exists in adhesion and migration during organogenesis. The intracellular domain of CD6 is constitutively phosphorylated, suggesting a role in T cell activation and signaling. In humans depletion of CD6-expressing cells increases graft versus host tolerance in transplants.

CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.

Specifications

• Antigen: CD6
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q61003
• Gene Symbols: CD6
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12511
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
• Clone: IM348
• Conjugate: Unconjugated
• Gene: CD6
• Gene Alias: Cd6; CD6 antigen; CD6 antigen, Tp120; Cd6 molecule; FLJ44171; OX52; Soluble CD6; T12; T-cell differentiation antigen CD6; TP120
• Host Species: Rat
• Quantity: 2 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a