CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 16-0541-050

Product Code. 17101052

Description

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody. Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis. YN1/1.7.4 has also been reported in blocking of CD54 in functional studies. Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD54 (ICAM-1) Monoclonal antibody specifically detects CD54 (ICAM-1) in Mouse samples. It is validated for Flow Cytometry, Functional Assay, Neutralization

Specifications

• Antigen: CD54 (ICAM-1)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P13597
• Host Species: Rat
• Quantity: 50 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: YN1/1.7.4
• Conjugate: Functional Grade
• Gene: ICAM1
• Gene Alias: BB2; CD54; cell surface glycoprotein P3.58; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; intercellular adhesion molecule-like; LOC100345640; Ly-47; Major group rhinovirus receptor; MALA-2; MyD10; P3.58; sCD54; soluble CD54
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15894
• Content And Storage: 4° C
• Form: Liquid