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CD5, APC-eFluor 780, clone: 53-7.3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-0051-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15539506

  • £174.66 / 100µg


EU Stock 4
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Description

Description

Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: This 53-7.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD5
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
P13379
CD5 antigen p56-62, T-cell surface glycoprotein CD5, lymphocyte antigen T1/Leu-1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
53-7.3
APC-eFluor 780
Cd5
Conjugated
Cd5
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
12507
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