CD5 Mouse, Clone: CD5/54/F6, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18112573	Novus Biologicals NBP2-32824-0.02MG	0.02 mg
18708053	Novus Biologicals NBP2-32824-0.1mg

Description

CD5 Monoclonal specifically detects CD5 in Human, Rat, Porcine, Bovine, Chicken, Equine, Guinea Pig, Monkey, Rabbit samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD5
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, SDS-Page
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Rat, Pig, Bovine, Chicken, Equine, Guinea Pig, Monkey, Rabbit
• Gene Accession No.: P06127
• Gene ID (Entrez): 921
• Immunogen: A synthetic peptide from the intracellular region of human CD5
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 67 kDa

• Clone: CD5/54/F6
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cell Biology, Cytokine Research, Immunology, Phospho Specific
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: CD5 antigen, CD5 antigen (p56-62), CD5 molecule, LEU1T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, T1
• Gene Symbols: CD5
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (∼10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.