CD49d (Integrin alpha 4), Functional Grade, clone: 9F10, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-0499-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 9F10 monoclonal antibody reacts with human CD49d, the 150kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).Applications Reported: The 9F10 antibody has been reported for use in flow cytometric analysis. 9F10 has also been reported in in vitro functional assays.Applications Tested: The 9F10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|CD49d (Integrin alpha 4)|
|PBS with no preservative; pH 7.2|
|269C wild type, CD49 antigen-like family member D, VLA-4 subunit alpha, antigen CD49D, alpha-4 subunit of VLA-4 receptor, ITGA4, ITGA-IV|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.