CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), Super Bright™ 702, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15923734	Invitrogen 67-0499-41	25 Tests
15933734	Invitrogen™ 67-0499-42	100 Tests

Description

Description: This CF1D12 monoclonal antibody reacts with human CD52, a cell surface glycoprotein expressed in the immune and reproductive systems. CF1D12 is thought to recognize the carbohydrate region of CD52, not the protein core of the molecule. Tissue-specific modifications of the glycan structure may alter epitope recognition in certain applications. For example, this CF1D12 clone has been reported to recognize human sperm glycoforms of CD52 by western blot, but not by flow cytometry. The immunogen used to generate this antibody was human CD52. Applications Reported: This CF1D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CF1D12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No.

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

Specifications

• Antigen: CD49d (Integrin alpha 4)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P13612
• Gene Symbols: ITGA4
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: ITGA4

• Clone: 9F10
• Applications: Flow Cytometry
• Conjugate: Super Bright 702
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3676
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody