CD49b (Integrin alpha 2) Monoclonal Antibody (HMa2), eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Invitrogen 14-0491-85

Product Code. 15266997

Description

Description: The HMalpha2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin alpha2 subunit. The complex of CD49b non-covalently associated with integrin beta1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4^+ T cells, IEL, NK cells, platelets and epithelial cells. Applications Reported: The HM alpha2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue (IHC-F). It has also been reported for use in in vitro functional assays (Please use Functional Grade purified HMa2, cat. 16-0491, in functional assays.). Applications Tested: The HM alpha2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of a-subunits (ai1, ai2 and ai3).

Specifications

• Antigen: CD49b (Integrin alpha 2)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q62469
• Gene Symbols: Itga2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16398
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: HMa2
• Conjugate: Unconjugated
• Gene: Itga2
• Gene Alias: BR, CD49B, GPIa, VLA-2, VLAA2, ITGA2
• Host Species: Armenian Hamster
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.