CD48, Functional Grade, clone: eBio156-4H9 (156-4H9), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-0489-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBio156-4H9 monoclonal antibody reacts with the human CD48 antigen, also known as B cell activation marker 1, SLAM family member 2, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. CD2 is the low affinity ligand for CD48.Recent publications have reported differential expression in mice of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors. Expression of CD48 in hematopoietic cells is found on many leukocytes with little to none on granulocytes and absent on platelets and erythrocytes. Expression increases on B cells with activation.Applications Reported: This eBio156-4H9 (156-4H9) antibody has been reported for use in flow cytometric analysis and functional assay.Applications Tested: This eBio156-4H9 (156-4H9) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48, CD48d|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.