BB700 Mouse Anti-Human CD45RO, Clone UCHL1 (RUO)

Mouse monoclonal antibody that specifically binds to the 180 kDa isoform of CD45 (aka, the Leukocyte Common Antigen).

Brand:  BD Biosciences 566521

Product Code. 15805129

Description

• The UCHL1 monoclonal antibody specifically binds to the 180 kDa isoform of CD45 (aka, the Leukocyte Common Antigen).
• CD45RO is a type I transmembrane glycoprotein that has cytoplasmic protein tyrosine phosphatase activity and functions in signal transduction pathways.
• This CD45 isoform does not include amino acid sequences encoded by the variable CD45 exons A, B, or C.
• CD45RO is expressed on most thymocytes, activated T cells, memory T cells, granulocytes and monocytes, but only on a proportion of resting T cells.
• CD45RO and CD45RA antibodies seem to define complementary, predominantly non-overlapping, populations in resting peripheral T cells, demonstrating heterogeneity within the CD8 and CD4 subpopulations.
• CD45RO binds to CD22.
• The antibody was conjugated to BD Horizon BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes.
• It is a polymer-based tandem dye developed exclusively by BD Biosciences.
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg., 695/40-nm filter).
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.

Specifications

• Antigen: CD45RO
• Classification: Monoclonal
• Conjugate: Brilliant Blue 700 (BB700)
• Quantity: 100 Tests
• Primary or Secondary: Secondary
• Target Species: Human
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: UCHL1
• Host Species: Mouse
• Regulatory Status: RUO
• Test Specificity: CD45RO
• Content And Storage: Refrigerate (2 to 8°C)