CD45RO Antibody (UCHL-1), Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NBP2-29631-20ug

Product Code. 18406921

Description

CD45RO Monoclonal specifically detects CD45RO in Human, Chimpanzee, Monkey samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Dual RNAscope ISH-IHC.

Specifications

• Antigen: CD45RO
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Flow Cytometry 0.5-1 ug/million cells, Immunohistochemistry 0.5-1 ug/mL, Immunocytochemistry/Immunofluorescence 1-2 ug/mL, Immunohistochemistry-Paraffin 0.5-1 ug/mL, Immunohistochemistry-Frozen 0.5-1 ug/mL, Dual RNAscope ISH-IHC
• Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
• Host Species: Mouse
• Molecular Weight of Antigen: 182 kDa
• Quantity: 20 ug
• Primary or Secondary: Primary
• Test Specificity: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: UCHL-1
• Conjugate: Unconjugated
• Gene Accession No.: P08575
• Gene Symbols: PTPRC
• Immunogen: Interleukin-2-dependent human T lymphocytes were used as the immunogen for the CD45RO antibody.
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 5788
• Target Species: Human, Primate
• Form: Purified

For Research Use Only