CD45R (B220), eVolve 605, clone: RA3-6B2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 83-0452-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RA3-6B2 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.|
For Research Use Only.