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CD45R (B220), PE, clone: YKIX753.22, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5451-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15567006

  • £157.52 / 100 tests


Estimated Shipment date
from Supplier 29-05-2018
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Description

Description

Description: The YKIX753.22 monoclonal antibody reacts with canine CD45R. In dogs, CD45R is expressed on B cells and peripheral T cells. In addition, CD45 exists as multiple isoforms due to alternative splicing. Some isoforms of CD45 are CD45RA, CD45RB, or CD45RO depending on the exon used during transcription. The C-terminal domain of CD45 exhibits tyrosine phosphatase activity that enables signaling from by T cell and B cell receptors following activation.This antibody has been reported to promote cell depletion via antibody-dependent cell-mediated cytotoxicity when used in conjunction with YKIX716.13.Applications Reported: This YKIX753.22 antibody has been reported for use in flow cytometric analysis.Applications Tested: This YKIX753.22 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.0075 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45R (B220)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Conjugated
PTPRC
IgG2b, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Canine
Flow Cytometry
YKIX753.22
PE
PTPRC
B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
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