CD45, eFluor 506, clone: HI30, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 69-0459-42

Product Code. 15500677

Description

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD45
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P08575
• Gene Alias: Cluster designation 45, common leukocyte antigen, T220 and B220
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow Cytometry
• Clone: HI30
• Conjugate: eFluor 506
• Gene: PTPRC
• Format: Conjugated
• Gene Symbols: PTPRC
• Isotype: IgG1, kappa
• Quantity: 100 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 5788

For Research Use Only.