CD45, clone: CD45-2B11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9457-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The CD45-2B11 monoclonal antibody reacts with human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in lymphocyte proliferation and differentiation. The 2B11 antibody is useful for recognition of normal and neoplastic lymphoid cells.Applications Reported: This CD45-2B11 antibody has been reported for use in flow cytometric analysis, immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This CD45-2B11 antibody has been tested by immunohistochemisty on formalin-fixed paraffin embedded tissue using high pH antigen retrieval. This can be used at less than or equal to 10 μg/mL. This CD45-2B11 antibody has also been tested by western blot at 5 μg/mL and by flow cytometric analysis on human peripheral blood cells at 0.25 μg/test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Cluster designation 45, common leukocyte antigen, T220 and B220|
|Flow CytometryþImmunocytochemistryþImmunohistochemistryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.