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CD44, PE-Cyanine5, clone: IM7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0441-83

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15340480

  • £213.39 / 200µg


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Description

Description

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD44
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P16070, P15379
CD44 antigen, Cell surface glycoprotein CD44, hyaluronate binding protein, ECMR-III, GP90 lymphocyte homing adhesion receptor, HUTCH-I, PGP-1, extracellular matrix receptor III, hermes antigen, hyaluronate receptor, phagocytic glycoprotein 1, phagocytic glycoprotein I
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
Flow Cytometry
IM7
PE-Cyanine5
CD44
Conjugated
CD44
IgG2b, kappa
200μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
960
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