CD44 Monoclonal Antibody (IM7), eFluor™ 506, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 69-0441-80

Product Code. 16351684

Description

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component. Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IM7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.

CD44 Monoclonal antibody specifically detects CD44 in Human, Mouse samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD44
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15379, P16070
• Gene Symbols: CD44
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12505, 960
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: IM7
• Conjugate: eFluor 506
• Gene: CD44
• Gene Alias: AU023126; AW121933; AW146109; BOS_15086; Cd44; CD44 antigen; CD44 molecule; CD44A; CDW44; CSPG8; ECMRIII; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; Heparan sulfate proteoglycan; HERMES; Hermes antigen; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; IN; LHR; Ly-24; Lymphocyte antigen 24; MC56; MDU2; MDU3; METAA; MIC4; PGP I; Pgp1; Pgp-1; PGPI; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; RHAMM; sCD 44; sCD44; soluble CD 44; soluble CD44
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b κ