CD43/Sialophorin Antibody (DF-T1), Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18198223	Novus Biologicals NBP2-15190-0.1MG	0.1 mg
18191644	Novus Biologicals NBP2-15190-0.2MG	0.2 mg
18449871	Novus Biologicals NBP2-15190-20ug	20 ug

Description

CD43/Sialophorin Monoclonal specifically detects CD43/Sialophorin in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD43/Sialophorin
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: CD43 antigen, CD43), Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, sialophorin (gpL115, leukosialin, CD43)
• Gene Symbols: SPN
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

• Clone: DF-T1
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: B Cell Development and Differentiation Markers, Immunology
• Gene Accession No.: P16150
• Gene ID (Entrez): 6693
• Immunogen: Myeloblastic KG1 cells were used as the immunogen for this antibody.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

For Research Use Only