CD43 Rat anti-Mouse, PE, Clone: eBioR2/60, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-0431-81

50UG ANTI-MOUSE CD43 PE

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15278529

Quantity Price
1 £ 88.0 / 50µg
Estimated Shipment date
from Supplier 24-02-2017
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Description and Specification

Specification

Antigen CD43
Format Conjugated
Gene Alias Leukosialin, LSN, Sialophorin, SPN
Applications Flow Cytometry
Isotype IgM
Quantity 50μg
Regulatory Status RUO
Species Reactivity Mouse
Primary or Secondary Primary
Clone eBioR2/60
Conjugate PE
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal or Polyclonal Monoclonal
Concentration 0.2mg/mL

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.