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CD43, FITC, clone: eBioR2/60, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0431-85

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15288079

  • £186.79 / 500µg


EU Stock 1
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Description

Description

Description: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.Applications Reported: This eBioR2/60 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD43
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P15702
CD43, GALGP, GPL115, LSN, galactoglycoprotein, leukocyte sialoglycoprotein, leukosialin, sialophorin gpL115, leukosialin, CD43
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBioR2/60
FITC
Spn
Conjugated
Spn
IgM
500μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
20737
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