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CD42a, eFluor 450, clone: GR-P, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0428-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15559676

  • £68.66 / 25 tests


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome.Applications Reported: Anti-Human CD42a eFluor® 450 has been reported for use in flow cytometric analysis.Applications Tested: The GR-P antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD42a
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P14770
CD42a, GPIX, GP-IX, glycoprotein 9, platelet glycoprotein IX, Platelet GPIX
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
GR-P
eFluor 450
GP9
Conjugated
GP9
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
2815
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