CD40/TNFRSF5, Rabbit anti-Human, Clone: A54 scFv, Novus Biologicals™

Rabbit Monoclonal Antibody

Product Code	Brand	Quantity
18289968	Novus Biologicals NBP2-81101-0.025MG	0.025 mg
18354090	Novus Biologicals NBP2-81101-0.2MG	0.2 mg

Description

CD40/TNFRSF5 Monoclonal specifically detects CD40/TNFRSF5 in Human samples. It is validated for ELISA, Immunocytochemistry/Immunofluorescence, Functional, Block/Neutralize.

Specifications

• Antigen: CD40/TNFRSF5
• Concentration: 1 mg/mL
• Applications: ELISA, Functional Assay, Blocking Assay
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Cancer, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
• Formulation: PBS
• Gene ID (Entrez): 958
• Immunogen: A54 human anti-CD40/TNFRSF5 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcgamma fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Clone: A54 scFv
• Dilution: ELISA, Functional, Block/Neutralize
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
• Gene Symbols: CD40
• Isotype: IgG λ
• Purification Method: Protein A purified
• Test Specificity: A54 binds specifically to the D1 domain of CD40/TNFRSF5 (and can only bind to the intact i.e. non-truncated version of CD40/TNFRSF5 and when an AD2 peptide tag has not been added) and can recognize CD40/TNFRSF5 on a B cell line (BJAB). This scFv binds to CD40/TNFRSF5 (immobilized by amine coupling) with a KD within the range of 40nM. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.